PARTS OVERVIEW
Basic Parts
Cell-penetrating Peptide - Part:BBa_K3090000
Nuclear localization signal (NLS) enriched in positively charged residues that can function as a Cell-penetrating peptide (CPP), similar to the classical CPP derived from HIV type 1 transactivator of transcription protein (HIV TAT).
Single Chain Variable Fragment (scFv(P5)) - Part:BBa_K3090001
scFv(P5) is a chimeric molecule in which several groups of residues important for antigen binding in the poorly stable anti-hen egg lysozyme (HEL) scFv(D1.3) were progressively grafted onto the scFv(F8) scaffold is to maintain cytoplasmic stability and specificity.
Composite Part
Single Chain Variable Fragment (scFv(P5)) with CPP - Part:BBa_K3090002
scFv(P5) is a chimeric molecule in which several groups of residues important for antigen binding in the poorly stable anti-hen egg lysozyme (HEL) scFv(D1.3) were progressively grafted onto the scFv(F8) scaffold is to maintain cytoplasmic stability and specificity. Cell-penetrating peptide from Porcine circovirus type 2 was fused to the N-terminus of scFv(P5) for cell penetration.